https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43200 Wed 22 Mar 2023 10:38:47 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44477 Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, were assessed for their ability in iron (Fe) removal from an AMD sample in nonimmobilized and immobilized systems. Use of free and immobilized cells exhibited 46−48% and 65−79% Fe removal, respectively, after 48 h of incubation. Compared with free cells, immobilized cells exhibited no apparent changes in morphology and granularity, as revealed by flow cytometry analysis, after their exposure to AMD samples. The second derivative spectra from Fourier transform infrared spectroscopy showed vibrational stretching for proteins and hydroxyl groups in immobilized cells. Thus, the immobilization technology offers a protective mechanism in acid-adapted strains against Fe present in AMD samples. Analysis of the immobilized acid-adapted microalgal technology by life cycle assessment (LCA) revealed its environmental sustainability because of less contribution to global warming and limited fossil fuel consumption. We demonstrated that the immobilized acid-adapted microalgal technology is much superior to calcined eggshell−microalgal or conventional limestone systems indicated in the literature for AMD treatment. Thus, this is the first study describing the potential application of microalgal cells entrapped in alginate beads in a greener and economical approach to treat AMD for sustainable mining.]]> Wed 07 Feb 2024 16:37:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37154 Desmodesmusv sp. MAS1 and Heterochlorella sp. MAS3, grown in synthetic acid mine drainage (SAMD). Virtually, there was no difference in the growth of the strain MAS3 both in Bold's basal medium (control) and SAMD. Using the IC₅₀ level (200 mg L^-1) and a lower concentration (50 mg L^-1) of iron in SAMD, the cell granularity, exopolysaccharide (EPS) secretion, iron recovery, and biodiesel were assessed in both the strains. Both cell granularity and accumulation of EPS were significantly altered under metal stress in SAMD, resulting in an increase in total accumulation of iron. Growth of the microalgal strains in SAMD yielded 12-20% biodiesel, with no traces of heavy metals, from the biomass. The entire amount of iron, accumulated intracellularly, was recovered in the residual biomass. Our results on the ability of the acid-adapted microalgal strains in iron recovery and yield of biodiesel when grown in SAMD indicate that they could be the potential candidates for use in bioremediation of extreme habitats like AMD.]]> Mon 24 Aug 2020 12:48:49 AEST ]]>